格隆溴铵杂质N用到的柱子有谁用过请教下？

psf161222

杂质N:色谱柱：默克 LichroCartChiraDex（4.0×250mm，5μm） 请问这根柱子怎么用啊？
说明书里有说保存
Column equilibration
LiChroCART® 250-4 ChiraDex® is supplied in methanol/water (40/60)
(v/v) and should be stored in the same solvent mixture. Before use, methanol/water (50/50) (v/v) should be pumped through the column for 30–60 minutes with a flow rate of 0.8 mL/min.，Conditions for chromatographic separations
分离条件：
For separations of enantiomers with ChiraDex®, mixtures of methanol
and water or buffer (methanol/water or buffer:5/95–60/40) (v/v) have been proven as well suited. The organic solvent plays an important role on enantioselectivity. The higher the polarity of the organic solvent (methanol > ethanol > acetonitrile), the better is
the separation of enantiomers. The separation of optical isomers canbe improved by using buffers (especially Na2HPO4/NaH2PO4) or 0.1%TEAA (triethyl amine acetate). The separation of enantiomers is depen-dent on the concentration of the buffer and on pH. The enantioselec-tivity of ChiraDex® can be altered by varying the temperature.
疑问：1.用完了怎么冲洗？2.纯乙腈纯甲醇能用吗？3. 这柱子有什么禁忌？